Extreme Physical Property of the Enzyme Layered Organization-The Functional Innovation by Using a Template of Layered Organization of Heat-Resistant and Low-Defect Organic / Inorganic Two-Dimensional Nano-Hybrid-

Abstract

Ultrathin films of regularly adsorbed biological molecules have been fabricated by means of the modified Langmuir-Blodgett (LB) method using an organo-modified montmorillonite (MMT). Adsorption of enzymes onto the anionic silicate surface could be confirmed by a comparison of the IR spectra of multilayers of organo-modified clay and those of lysozyme-adsorbed films. The IR bands of lysozyme were clearly confirmed in the spectra of enzyme-adsorbed multilayers. Enzyme activity of the enzyme-adsorbed multilayers was maintained at high temperatures, up to 160 °C. This suggests that three-dimensional structure of enzyme inserted in clay particle were maintained at high temperature.