Abstract
The objective of this study is to manufacture novel magnetic polymer nanoparticles with phospholipid polymer brush layers and functional groups to regulate the interactions with intercellular biomolecules or cells. The combination of the magnetic polymer nanoparticles and polymerase chain reaction technique would lead to the understanding of intracellular molecular reactions. In this study, we designed the polymer nanoparticles possessing a remote controllability and biomolecules-binding ability. Remote controllability was accomplished by encapsulating magnetic nanoparticles in the polymer nanoparticles via miniemulsion polymerization of styrene with Fe3O4 nanoparticles. To prevent the non-specific interaction with intracellular biomolecules, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) brush layer was constructed on the nanoparticles by surface-initiated atom radical polymerization. The magnetic polymer nanoparticles were specifically transferred into cells by immobilizing cell-penetrating peptide at the end of poly(MPC) chains. In particular, the applied external magnetic field enhanced cellular uptake.
