Abstract
The usefulness of enzymatic HbA1c measurement was studied in view of international HbA1c standardization. Norudia N HbA1c (Sekisui Medical Co. Ltd.) was used for enzymatic method and the resulting value was compared to that by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) , HPLC, and immunological method. Results showed that: (1) raw data before correction by the Japan Diabetes Society (JDS) Lot 3 calibrator obtained by the enzymatic method were almost the same as that by the IFCC method, (2) When HbA1c values corrected by JDS Lot 3 were compared enzymatically and HPLC or immunologically, 0% obtained enzymatically corresponded to 0.2 to 0.3% by HPLC, unlike immunologically, which was almost 0%, (3) regression line analysis of raw data compared enzymatically and by HPLC showed the two to differ slightly. Enzymatic HbA1c measurement is therefore useful in the international standardization of HbA1c measurement.
