Abstract
When the Liver slices were incubated with glucose, the tissue glucose content and the output of glucose from tissue were increased in the diabetes more than that of the normal. The addition of xylitol or insulin to the diabetes caused a decrease of glucose output.
The incorporation of glucose-U-14C into glycogen in the diabetes was simulated by the addition of xylitol. During the incubation for 1.5 hours, glucose-6-phosphate content increased twice, and uridinediphosphate glucose decreased to about a third by the addition of xylitol.
The glycolytic intermediates were also determined by the enzymatic micoromethod. In the diabetic tissue, the concentration of glycolytic intermediates, and ATP, ADP and NAD were lower than those in the normal. The addition of xylitol to the diabetes caused the increases of the intermediates ofglycolysis, and particularly, the intermediates from triose-phosphate to pyruvate. The free energy change between glucose and pyruvate were -15.7 kcal/mole in the normal and -17.8 kcal/mole in the diabetes. With addition of xylitol to the diabetic liver slices, free energy change in glycolysis was -16.6 kcal/mole, and free energy change from glyceraldehyde phosphate to pyruvate became to small (-6.49→4.92 kcal/mole)
Xylitol would be penetrated into the tissue without any relation to insulin and exert the positive feedback on glycolysis via Pentose Phosphate Cycle.
