Abstract
There are two methods for the determination of plasma insulin by immunoassay: a single antibody And a double antibody method.
The single antibody assay is theoretically more desirable than the two antibody method, however, almost all methods of the single antibody immunoassay seemed more complicated than those of double antibody assay.
In this paper, a new method using ethanol precipitation is described for the assay of plasma insulin. In this procedure, labeled insulin bound with antibody is precipitated in the presence of ehtanol (terminal concentration of ehtnol: 67%) at 4°C, unbound labeled insulin remaining in solution.
This method is simple and seems sufficientyl available to determine plasma insulin.
