Abstract
A simple method for separation of plasma chylomicron and colorimetric determination of its triglyceride content was described.
Chylomicron in plasma, diluted appropriately, was collected by ultra-filtration using cellulose nitrate membrane filter with pore size of 100mμ. Chylomicron in residue was extracted with chloroform and triglyceride-glycerol was measured. The mean recovery rate of chylomicron-triglyceride was 88.6%. The mean relative error was 7.6% and there was no loss of accuracy for plasma from patients with gross hyperchylomicronemia.
An analysis using paper electrophoresis revealed validity of chylomicron separation by the filtration method.
The mean value of fasting chylomicron-triglyceride was 4.8±1.1 mg/100 ml (± SE) in 15 normal subjects, 12.2±2.0 mg/100 ml in 18 moderate diabetics and 30.9±5.0 mg/100 ml in 13 severe diabetics.
