Abstract
The effect of tolbutamide on the oxidation and phosphorylation of the isolated rat liver mitochondria was tested, and following results were obtained.
1. More than 30 mg% tolbutamide increased the state 4 respiration rate and decreased respiratory control of isolated rat liver mitochondria.
2. More than 80 mg% tolbutamide decreased the state 3 respiration rate.
3. 100 mg% tolbutamide decreased P/o ratio to zero.
4. 100 mg% tolbutamide increased apparently rutamycin sensitive ATP-ase activity of rat liver mitochondria.
Above mentioned results showed that tolbutamide was one of uncouplers. This results were discussed in relation to the side effect of tolbutamide.
