Abstract
Serum lipids, postheparin triacylglycerol lipase (PHTGL) and postheparin monoacylglycerol hydrolase (PHMGH) activities in rats with streptozotocin-induced diabetes (80mg/kg) were measured. Release of triacylglycerol lipase and n.cncacy: E, cerol 1.yc: Iclase in vArc frcrn slices of liver and adipose tissue in these rats were also measured.
Following results were obtained:
1) Serum triacylglycerol and free fatty acid (FFA) levels were significanty higher in the diabetic than the control rats. However, serum phospholipid and cholesterol levels were not significantly different between these two groups.
2) PHTGL and PHMGH activities were significantly lower in the diabetic than the control rats.
3) In vitro release of triacylglycerol lipase from the adipose tissue in diabetic rats was significantly smaller than in the control rats.
4) In vitro release of monoaylglycerol hydrolase from the liver was markedly decreased in diabetic rats.
5) The increase in serum triacylglycerol (TG) and FFA and the decrease in PHTGL and PH MGH activities in the diabetic rats were significantly inhibited by the administration of insulin.
These results suggest that the increase of the serum TG in diabetic rats was caused the reduced PHTGL activity of the adipose tissue probably due to the insulin deficiency.
