Abstract
A simple in vitro method for detection of igE insulin antibody using the principle ofradioallergosorbent test (RAST) is described.
In 6 patients with insulin allergy, the RAST values were higher than those of normal persons or insulin-treated diabeatic patients without insulin allergy. No difference was observed between normal persons and insulin-treated diabetic patients without insulin allergy.
One patient with insulin allergy showed a gradual decrease of the RAST value corresponding to IgG insulin antibody and clinical allergic syndromes after treatment with Monocomponent insulin.
This method, though it is slightly inhibited by non-IgE insulin antibody and issemi-quantitative, is reproducable and simple. It is, therefore, useful for the detection of IgE antibody against insulin
