Abstract
An immunochemical method was developed to measure two different triglyceride lipase activities in rat postheparin plasma: hepatic triglyceride lipase (H-TGL) and extrahepatic lipoprotein lipase (LPL). The two lipases were separated by affinity chromatography using Sepharose covalently linked heparin. The H-TGL thus separated and partially purified was injected into a rabbit and the antiserum against this enzyme was produced. Use of this antiserum then permitted specific measurement of the two lipases in whole postheparin plasma. Although the exact roles of the two lipases in lipoprotein metabolism have not yet elucidated, the present method appears to represent an important technique for studying the pathogenesis of experimental hypertriglyceridemia.
