Clinical Significance of the Determination of HbA_I Components

Abstract

The components of the HbA_I fraction were determined by high-performance liquid chromatography using both phosphate buffers with pH 6.65 and pH 6.45 without containing KCN. The HbA_Ia+b and HbA_Ic components were expressed as percentages of the total Hb.
The HbAic component occupied a greater part of the HbA_I in diabetic patients than in normal subjects. Although patients with renal failure showed higher HbA_I values, no difference in the ratio of the components in HbA_I. was noted between these patients and normal subjects. Determinations of the HbA_I components of young and old erythrocytes indicated that HbA_Ic component occupied a greater part of the HbA_I in old than in young erythrocytes. Quantification of the HbA_I content revealed that the HbA_I fraction fluctuated rapidly through rapid change of blood glucose concentration and it was mainly HbA_Ic component.
The above results sggest that determinations of HbAi components should be carried out in various pathophysiological conditions.