Abstract
Several reports have indicated that a form of hyperglucagonemia, which reacts with antibody specific to pancreatic glucagon, occurs in pancreatectomized and eviscerated rats. The kidney is suspected to be one of the sites of origin of glucagon other than the pancreas and gastrointestinal tract.
To detect whether the kidney releases glucagon, rat kidneys were perfused in vitro with 20 mM arginine or 10 ng and 100 ng isoproterenol, which are sufficient to release glucagon from perfused rat pancreas. The perfusates were assayed for glucagon using 30 K, but no increase of glucagon was detected.
It was also found impossible to extract glucagon from the rat kidney tissue.
