Abstract
To estimate the glucose metabolism of the myocardium in a diabetic state and to study the effect of coenzyme Q10 (CoQ10) on the myocardial metabolism of glucose, we observed glucose uptake, lactate production and glucose oxidation in the isolated perfused heart from streptozotocin (STZ)-incluceJ diabetic rats and CoQ10-treated diabetic rats.
Male Wistar rats weighing 120g were intraperitoneally administered with STZ 60mg/kg body weight, and olc-half of the rats were intraperitoneally injectd with CoQ10 10mg/kg twice a day for one week. The heart was isolated and perfused at a coronary flow rate of 2ml/min with a synthetic medium containing 1 mM glucose and [1-14C]-glucose for 30 min.
In the control rats, glucose uptake, lactate production and glucose oxidation were 9.0±0.8μrnol/30min/g wet ti ;MC weight (mean±SD), 40.5±12.4μmol/30min/g and 22.9±2.3nmol/30min/g, respectively. Glucoie uptake (4.4±2.6μmol/30min/g), lactate production (25.0±6.8μmol/30min/g) and glucose oxidation (22.0±7.8nmol/30 min/g) in diabetic rats were significantly (P<0.01) lower than those in controls. Glucose uptake (4.6±2.8μmol/3 min/g) and lactate production (23.7±4.8μmol/30 min/g) in CoQ10-treated diabetic rats were similar to those in diabetic rats, but glucose oxidation (30.7±5.2 nmol/30min/g) was significantly increased (p<0.05).
