Abstract
To determine the direct effect of arterial glucose concentration on urinary excretion of N-acetyl-β-D-glucosaminidase (NAG), NAG activity was measured in the urine from rat kidneys vascularly perfused for 40min with a synthetic medium containing 5.5mmol of glucose, 16.7mmol of glucose, or 5.5mmol glucose and 11mmol of mannitol. Urinary NAG activity in the kidney perfused with 16.7mmol of glucose (2.9±1.3mU/40min, mean±SD) was significantly greater than that (0.5±0.1mU/40min or 0.6±0.2mU/40min) perfused with 5.5mmol of glucose or 5.5mmol of glucose and 11mmol of mannitol, respectively.
These results suggest that urinary NAG excretion is strongly influenced by arterial blood glucose concentration.
