Abstract
In this report insulin neutralizing activities of antisera of guinea pigs and rabbits, and change of serum ILA before and after glucose loading in several diseases with metabolic abnormality of glucose were studied. Serum ILA was measured with technique by Renold et al. and indicated as glucose uptake mg/g of epididymal adipose wet tissue weight.
Insulin standard curve; In following concentration, from 15 to 1000 microunits/ml, response curve was linear (λ=0.35). Guinea pigs were immunized with insulin Freund's adjuvant, and rabbits with insulin bis-diazo-benzidine (BDB) sheep erythrocytes.
It had shown that all of antisera of guinea pigs had high neutralizing activities, max. 1000 microunits/ml, in vitro with this method, but of rabbits showed less neutralizing activities, however high BDB hemaggultination titres. Peritoneal injection of antisera of guinea pigs caused markedly hyperglycemic reactions in Wister rats.
Studies on change of serum ILA in several diseases;
i) In diabetes, after glucose loading, bloodsugar curve was hyperglycemic and the time of peak of serum ILA was late and/or showhd low responsiveness, especially in juvenile types and some cases with ketosis.
ii) In acromegaly, showing normal bloodsugar levels, serum ILA in both fasting and after glucose loading were high.
iii) In steroid diabetes, serum ILA was low and/or peak was so late as diabetes mellitus.
iv) In hyperthyroidism, no clear change of serum ILA or correlation between bloodsugar and serum ILA was shown.
v) In addison disease and hypophyseal insufficiency relative hypoglycemia and low serum ILA were observed.
vi) Langerhans islet cell adenoma showed high value of fasting serum ILA with hypoglycemia and hypoglycemic coma after intravenous Tolbutamide test, then bloodsugar level fell down markedly to 18mg/dl
