1984 Volume 30 Pages 11-16
The preservation of cells and activities of the bacterial strains by L-drying and freeze-drying methods followed 14 day preservation tests at 37℃ were investigated. The cells of nicotine degrading bacteria, Arthrobacter globiformis JTS-6, more survived by L-drying than the cells preserved by freeze-drying, and the survived cells of Pseudomonas putida JTS-9 were twice as many as those by freeze-drying. The nicotine degrading activities of the preserved cells of the two strains were the same as those of original cells, respectively. In the case of the bacterial strain which improves the taste and aroma of cured tobacco leaves, Bacillus pumilus JTS-308, the viable cell number after L-drying was 73% of that after freeze-drying, but its activity for tobacco leaves was completely preserved. The cells preserved by L-drying of the pathogenic bacteria of tobacco bacterial wilt, Pseudomonas solanacearum JTS-338, survived ten time as many the survived cells preserved by freeze-drying, and 100% of the slime forming activity (pathogenicity) was also preserved.
