1986 Volume 32 Pages 78-82
Effect of cryoprotectants on the preservation of viability and activities of the bacterial cells at -100℃ freezing for one year was investigated. Using 10% glycerol, cells and activities of Arthrobacter globiformis JTS-6 (nicotine degrading bacterium), Pseudomonas putida JTS-9 (nicotine degrading bacterium), Pseudomonas solanacearum JTS-338 (virulent strain of Bacterial Wilt) and P. solanacearum JTS-340 (avirulent strain of P. solanacearum JTS-338) were well preserved. The viability of P. solanacearum JTS-338 and JTS-340 was inhibited with 10 and 20% DMSO. P. solanacearum JTS-338 and JTS-340 were well preserved by L-drying and -100℃ freezing methods for long periods. They could also be preserved in distilled water for 4 months at 20℃ without mutation. Avirulent strain of P. solancearum was more proof against the cold and dryness than the virulent one.
