Journal of Toxicologic Pathology
Online ISSN : 1881-915X
Print ISSN : 0914-9198
ISSN-L : 0914-9198
Short Communication
Immunohistochemical Expressions of Main PGE2 Biosynthesis-related Enzymes and PGE2 Receptor in Rat Nephrogenesis
Emi YamamotoTakeshi IzawaMitsuru KuwamuraJyoji Yamate
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Keywords: rat, nephrogenesis, PGE2, COX-2, EP4

2011 Volume 24 Issue 4 Pages 257-261


Endogenous prostaglandin (PG) E2 plays important roles in renal homeostasis. Immunoexpressions of PGE2 biosynthesis-related enzymes, cyclooxygenase (COX)-2 and microsomal PGE2 synthetase (mPGES)-1 and EP4 (a PGE2 receptor), were investigated in renal development. Kidney tissues were obtained from fetuses on gestation days 18 and 21 and neonates on days 1 to 18. In fetuses and early neonates, the expressions of COX-2, mPGES-1 and EP4 were observed in developing renal tubules, indicating that COX-2 and its product, PGE2, play important roles in blastemal cell-derived renal tubular development via EP4. Cyclin D1 expression was seen in both the nucleus and cytoplasm of the developing tubules. These findings differed from the decreased COX-2 expression and exclusive nuclear expression of cyclin D1 seen in abnormal epithelial regeneration of injured renal tubules in cisplatin-treated rats in our previous articles. Collectively, PGE2, induced by COX-2, regulates renal tubular epithelial formation via EP4.

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© 2011 The Japanese Society of Toxicologic Pathology
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