Journal of Toxicologic Pathology
Online ISSN : 1881-915X
Print ISSN : 0914-9198
ISSN-L : 0914-9198
Technical Report
Databases for technical aspects of immunohistochemistry: 2021 update
Takayasu MorokiSaori MatsuoHirofumi HatakeyamaSeigo HayashiIzumi MatsumotoSatoshi SuzukiTakashi KoteraKazuyoshi KumagaiKiyokazu Ozaki
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JOURNALS OPEN ACCESS

2021 Volume 34 Issue 2 Pages 161-180

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Abstract

With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) as well as the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, blocking condition, secondary antibody information, etc.) for a total of 509 primary antibodies (comprising 220 different types). These survey results were an update on the contents reported by CEAH in 2017.

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© 2021 The Japanese Society of Toxicologic Pathology
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