Abstract
Two subcutaneous transplantable rat osteosarcoma lines were established serially and used to analyze the mechanism of radiographic sclerotic changes in human osteogenic sarcomas following chemotherapy. Primary osteogenic sarcomas were induced in the left humerus (POA) and in the right femur (POB) by [32P]H3PO4 in one F344 male rat. Pieces of POA or POB tumors measuring about 1mm3 were transplanted subcutaneously into syngeneic male rats up to the 22nd generation, for a period of three years and six months. Both the POA and POB lines maintained osteoid and bone formation, and alkaline phosphatase (ALP) activity. Moreover, tumor nodules grown after transplantation of the POA or POB line showed severe radiographic sclerotic changes three or four weeks after a single dose of cis-diamminedichloroplatinum (CDDP). Histologically, the sclerotic changes were caused by increased bone formation.
