LACK OF EFFECTS OF CHOLECYSTOKININ AND PROGLUMIDE, ANTAGONIST OF ITS RECEPTOR, ON PANCREATIC DUCTAL CARCINOGENESIS INDUCED BY N-NITROSOBIS (2-HYDROXYPROPYL) AMINE IN HAMSTERS

Abstract

The effects on pancreatic carcinogenesis of cholecystokinin (CCK) and/or proglumide (PGL), antagonist of CCK receptor, were studied in male hamsters initiated with N-nitrosobis (2-hydroxypropyl) amine (BHP). The animals were initially given weekly 500mg BHP/kg body weight subcutaneous injections for 5 times. This was followed by 40IDU/kg body weight by subcutaneous injections of CCK 3 times a week and/or by 1.25% or 2.5% PGL in the diet for 30 weeks. Pancreatic lesions including duct epithelial cell hyperplasia and atypical hyperplasia, intraductal carcinomas, and invasive carcinomas were induced in all groups of hamsters given BHP. However, no significance influence of CCK and/or PGL on the induction of these lesions was observed. Furthermore, CCK and/or PGL did not affect DNA synthesis in their component cells as judged by BrdU immunohistochemistry.