INDUCTION OF COLON ADENOCARCINOMAS BY 1, 2-DIMETHYLHYDRAZINE IN (C3H×MSM) F₁ MICE

Abstract

The possibility of producing colon tumors by administration of 1, 2-dimethylhydrazine (DMH) to (C3H×MSM) F₁ hybrids of inbred and wild strains mice, was investigated in an attempt to establish an experimental model for detection of DNA polymorphism markers for colon carcinogenesis in mice. DMH at doses of 0, 10, and 15mg/kg body weight was given intra-peritoneally once a week for 10 weeks and the experiment was terminated 46 weeks after the initial administration. The first colon tumors appeared after 30 weeks and final incidencs reached 38.5% in female mice receiving 10 mg DMH and 90.9 and 100%, respectively, in male and female mice receiving 15mg DMH/kg body weight. Tumors were multiple, mainly located in the distal colon and primarily adenomas and adenocarcinomas.