Abstract
This poster is the 3rd part of our positive- and vehicle-control validation studies in murine local lymph node assay (LLNA). Based on our vehicle-control test results, ethanol-water systems were found to be suitable for LLNA tests. One of the most useful vehicles is the ethanol water (70/30, v/v)(EtOH 70%) solution.
In order to provide sufficient scientific rationale, a further investigation and comparison of the allergenic potency and local clinical signs caused by the positive control substance alpha-hexylcinnamaldehyde (HCA) have been made. In 6 parallel LLNA validation studies wiht 96 CBA/CaOlaHsd mice performed at RCC, 6 different solvent systems, 5 of them recommended by OECD Guideline 429, have been assayed.
In order to rationalize and quantitatively analyze the test results, some relevant physiochemical properties of the 6 organic solvents have been compared, and some statistic analyses have also been performed with the data obtained from 6 different vehicle systems.
