Abstract
In this study, 6 main study groups of 10 F344 rats/sex were given 0,300,600,900,1200,1500mg/kg Myristica Fragrans Houttuyn for 90 days. Special endpoints in this study were included sperm morphology and vaginal cytology. All animals survived to the scheduled termination. In clinical signs, there were treatment-related salivation at dosage of 600, 900, 1200 and1500mg/kg after injection. Irregular estrous cycle and long anestrous period were observed in 900, 1200, 1500mg/kg treatment group. Absolute organ weights of liver were increased in male 1200, 1500 and female 900, 1200, 1500mg/kg treatment group. Necropsy findings showed uterus atropy in female 1500mg/kg treatment group. Histopathological findigs showed basophilic tubular change of kidney in all male treatment group compared than control group. In liver, mild cellular infiltration, microgranuloma, necrosis, hyperplasia, sinusoidal dilatation were showed dose-dependently in male and female treatment group. Atropy of uterus and ovaries were showed dose-dependently in female treatment groups. Based on these results, NOEL is considered to be 300mg/kg in male and female.
