Abstract
This study was designed to investigate the effect of boiled Moringa oleifera LAM pod (bMO) on azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted two stage mouse colon carcinogenesis in the promotion stage. Male ICR mice were divided into 8 Groups. Group 1 was served as a negative control group. Group 2 was received AOM (10 mg/kg BW, i.p.), followed by DSS (2% in drinking water for one week), and served as a positive control group. Groups 3-5 were fed with 1.5%, 3.0%, and 6.0% bMO, respectively supplemented in a basal diet after AOM/DSS administration, for 15 weeks. Groups 6-8 were fed with only 1.5%, 3.0%, 6.0% bMO, respectively in basal diets for 15 weeks. At the end of the study we found that the incidences and multiplicities of tumor in Groups 3-5 were decreased from positive control but significantly only in Group 3. PCNA index in Groups 3 and 4 was also significantly decreased, compared with those of the positive control group, while iNOS expression was significantly decreased in all doses of bMO. However, there was loss of COX-2 expression in all doses of bMO. The results suggest that M. oleifera LAM pod has potentially suppressive effect against colitis-related colon carcinogenesis induced by AOM/DSS. The lower dose is more effective at the promotion stage than at higher doses.
