A Combined, affordable solution for the performance of automated 3D in vitro hepatotoxicity testing

Abstract

To meet the demand for increased hepatotoxicity testing, automation has been incorporated to streamline the procedure and reduce the need for large scale manual manipulations. Here we demonstrate the ability to combine liquid handling with a novel cell imaging multi-mode reader to perform 3D hepatotoxicity studies. hiPSC-hepatocytes were aggregated into spheroids easily and efficiently using magnetic bioprinting technology. Multiple known hepatotoxicants were tested with the automation and cell model to validate the ability of the combined solution to be used to meet the need to perform automated, walk-away hepatotoxicity studies in an academic setting.