Single cell-ICP質量分析計を用いた単一細胞中の元素分析法の開発

田中 佑樹; 飯田 里紗子; 久保田 哲央; 山中 理子; 杉山 尚樹; 小椋 康光

doi:10.14869/toxpt.47.1.0_O-16

The 47th Annual Meeting of the Japanese Society of Toxicology

Session ID : O-16

DOI https://doi.org/10.14869/toxpt.47.1.0_O-16

Conference information

Host: The Japanese Society of Toxicology

Name : The 47th Annual Meeting of the Japanese Society of Toxicology

Date : 2020 -

Oral

Elemental analysis of single cell using ICP mass spectrometry

*Yu-ki TANAKA, Risako IIDA, Tetsuo KUBOTA, Michiko YAMANAKA, Naoki SUGIYAMA, Yasumitsu OGRA

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CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract

Recent advances in analytical techniques using an ICP-MS enable us to detect a trace or ultratrace amount of element in a single cell at a level of femtogram or attogram. In this study, we measured elemental contents in a single cell such as red blood cell, yeast, green algae, and human leukemia cell (K562) by a single cell-ICP-MS. The measured elemental contents of Mg, Zn, P, S, and Fe showed good agreements with the values obtained from conventional solution based ICP-MS analysis after acid digestion of the cells. This analytical technique can be useful strategy for evaluating the metabolism of both endogenous and exogenous elements in a single cell.

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