Host: The Japanese Society of Toxicology
Name : The 47th Annual Meeting of the Japanese Society of Toxicology
Date : 2020 -
The water flea Daphnia magna, is a branchiopoda crustacean, which is a common inhabitant in fresh water. In general, normal and healthy populations are entirely female because they produce by parthenogenesis. Previously we and others found that females exposed to juvenile hormone produce males, suggesting that juvenile hormone is a key molecule for sex determination. By using genome editing tools, we developed transgenic lines that can detect hormone like activities by expression of fluorescent protein. One line has ecdysone response elements in the promoter region of fluorescent protein and the other has juvenile hormone response elements in its promoter. These transgenic lines enabled us to detect spatiotemporal distribution of ecdysone and juvenile hormone. In addition, these lines also could detect hormone like activities present in aquatic environment. As there was no convenient system for juvenile hormone activities to date, these lines are useful for the evaluation of hormone like activities.
