Host: The Japanese Society of Toxicology
Name : The 47th Annual Meeting of the Japanese Society of Toxicology
Date : 2020 -
Adverse effects of CNS are a major safety concern on the drug development. We tried Ca-transient assay using iCell DopaNeurons and XCell Neurons. Cells were cultured in 96well U-bottom plates. Calcium sensitive fluorescence indicator (EarlyTox Cardiotoxicity Kit) and test compounds (4-aminopyridine and so on) were added and Ca-oscillations were measured with FDSS/µCELL. This simple and effective throughput assay detected the effects of neurotoxic substances. Gabazine induced better response in XCell Neurons (Glutamatergic/GABAergic). We will also show our scheme for practical use.