Annual Meeting of the Japanese Society of Toxicology
The 47th Annual Meeting of the Japanese Society of Toxicology
Session ID : P-136
Conference information

Poster
Trials for simple neuronal Ca-transient analysis using human iPS cell-derived neurons to assess the toxic effects on central nervous system
*Takuya KONDOShohei KANIEKazuhiko BESSHI
Author information
CONFERENCE PROCEEDINGS FREE ACCESS

Details
Abstract

Adverse effects of CNS are a major safety concern on the drug development. We tried Ca-transient assay using iCell DopaNeurons and XCell Neurons. Cells were cultured in 96well U-bottom plates. Calcium sensitive fluorescence indicator (EarlyTox Cardiotoxicity Kit) and test compounds (4-aminopyridine and so on) were added and Ca-oscillations were measured with FDSS/µCELL. This simple and effective throughput assay detected the effects of neurotoxic substances. Gabazine induced better response in XCell Neurons (Glutamatergic/GABAergic). We will also show our scheme for practical use.

Content from these authors
© 2020 The Japanese Society of Toxicology
Previous article Next article
feedback
Top