Tissue clearing by RAP system is available for immunohistochemistry

Abstract

We previously reported a rapid bone staining procedure (RAP-B) based on a rapid and nondestructive tissue clearing system (RAP system), preserving the histological structure of tissues even after the specimens were highly cleared. In the present study, we applied the RAP system to immunohistochemical observation. The RAP system improved permeation of antibodies into whole-mount specimens and thick tissue sections. Thus, we concluded that tissue clearing by RAP system could provide the great advance in immunohistochemical analysis.