メフェナム酸による肝障害を減弱させるヒト肝臓酵素の同定

深見 達基; 小木曽 巧朗; Zhongzhe CHENG; 小西 慶吾; 中野 正隆; 中島 美紀

doi:10.14869/toxpt.47.1.0_P-208

The 47th Annual Meeting of the Japanese Society of Toxicology

Session ID : P-208

DOI https://doi.org/10.14869/toxpt.47.1.0_P-208

Conference information

Host: The Japanese Society of Toxicology

Name : The 47th Annual Meeting of the Japanese Society of Toxicology

Date : 2020 -

Poster

Superoxide dismutase 1 attenuates reactive quinoneimine formation from mefenamic acid in the human liver

*Tatsuki FUKAMI, Takuo OGISO, Zhongzhe CHENG, Keigo KONISHI, Masataka NAKANO, Miki NAKAJIMA

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CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract

The purpose of study is to identify hepatic enzyme(s) responsible for the decrease in the formation of mefenamic acid-quinoneimine (MFAQI), which may be a reactive metabolite of mefenamic acid. The CYP1A2-mediated MFAQI formation was significantly decreased by human liver cytosol. Superoxide dismutase 1 (SOD1) was identified, by purification from cytosol, as an enzyme decreasing MFAQI. Cytotoxicity by MFA in CYP1A2-overexpressed HepG2 cells was enhanced by knockdown of SOD. Thus, we found that SOD1 has a role to decrease MFAQI production, which may be due to scavenging of superoxide.

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