Development of culture conditions for repeated dose toxicity test and cholestasis toxicity test

Abstract

In this study, the conditions for long term culture and extended canaliculi formation were examined in human-induced pluripotent stem cell-derived hepatocytes (hiPSC-hep) in order to evaluate for the repeated dose toxicity and the cholestasis toxicity.

When hiPSC-hep from vendor A were cultured in long-term culture medium from vendor B, the gene expression levels of CYPs and ALB were maintained for 28 days. In addition, culture condition for forming equally the extended-canaliculi on the entire culture surface was successfully established.