Toxicity evaluation by high-content analysis of 3D cultured primary human hepatocytes

Abstract

DILI is one of the major reasons for drug discontinuation and withdrawal from the market, therefore highly predictive toxicity model is desired.　The development of liver toxicity is mediated by a variety of mechanisms, making it difficult to predict from a single biomarker. In these circumstances, we attempted to improve in vitro model for DILI detection by conducting HCA using 3D culture of human primary liver cells as a study to construct a liver toxicity model. Cells were exposed to multiple drugs causing DILI and were comprehensively evaluated using various toxicity biomarkers.