Investigation of analytical method focusing on a multivariate analysis for a seizure risk assessment using in vitro MEA systems

Abstract

Seizure is one of the serious toxicities in drug development. Recently, in vitro study using cultured neurons and microelectrode array (MEA) systems showed the potential to detect a seizure risk of compounds inducing seizure in vivo, by using parameters based on neuronal activities. However, these analytical methods are not well established. The purpose of this study was to　investigate the analytical method focusing on a multivariate analysis using a number of parameters calculated by MEA systems, in order to categorize test compounds into seizurogenic or non-seizurogenic. As a result, cluster analysis was able to classify the seizurogenic compounds and non-seizurogenic compounds.