Hepatocarcinogenesis with NASH in mice fed a choline-deficient, methionine-lowered, amino acid-defined, high fat diet (CDAA-HF-T (-))

Abstract

NASH causes liver cirrhosis and cancer. In order to elucidate its underlying mechanism, we have developed a NASH model of mice fed a CDAA-HF-T (-) diet. In this study, 6-week-old male C57BL/6J mice were fed the diet for 14 months. As a result, multiple giant nodules were observed in the livers of all mice at the end of month 12, and hepatocellular carcinomas and hemangiosarcomas were induced at the end of month 14. In conclusion, this model is useful to reveal details of NASH-associated hepatocarcinogenesis in mice.