Results and challenges in subretinal injection in rats, rabbits, and cynomolgus monkeys

Abstract

Subretinal injection is widely used in gene therapy research on age-related macular degeneration and retinitis pigmentosa. Rats, rabbits, and cynomolgus monkeys are often used, but the maximum dose volume and technique must be set in consideration of species. We subretinally administered saline to rats and monkeys and examined IOP fluctuations immediately after dosing. IOP increased up to 2–fold in rats administered 5 μL for 2 seconds, but did not increase in rats administered 5 μL for 1 minute. We also examined the maximum dose volume and IOP changes in monkeys and will present the results.