Cell-to-cell variations of human iPS-derived cardiomyocytes

Abstract

Human iPS cell-derived cardiomyocyte (hiPSC-CM) is conceptually promising as an unlimited source of human cardiomyocytes for pre-clinical safety testing. However, intra- and interline variations in functional properties remain to be solved completely. In order to quantify the variations, we employed a motion field imaging to assess the contractile functions. The technique revealed cell-to-cell variations of contractile function of hiPSC-CMs. We would like to discuss on the impact of the variation on pharmacological/toxicological assessment.