Host: The Japanese Society of Toxicology
Name : The 47th Annual Meeting of the Japanese Society of Toxicology
Date : 2020 -
CRISPR/Cas9 system has opened the new era for reverse genetics. In 2013, we developed the efficient gene knockout system in mice by injecting the plasmids expressing humanized Cas9 (hCas9) and single-guide RNA (sgRNA) into zygotes. Now it is replaced by electroporation of oocytes with CAS9/crRNA/tracrRNA ribonucleoprotein complex for simple genome editing (knockout, point mutation, tag insertion, etc). Altogether, to date, we have knocked out 272 testis abundant genes and analyzed the phenotypes in vivo. Whereas 168 of the KO mouse lines were fertile and did not show any drastic phenotypes, 9 KO mouse lines showed lethality. The remaining 103 KO mouse lines showed infertility or subfertility and propelled our research. I would like to introduce recent findings in mammalian fertilization. In addition, I would also like to discuss on the risk and benefit of CRISPR/Cas9 mediated genome editing in mice.
