High-throughput alternative method to developmental toxicity test based on the accumulation of RTK-SRF signal disruption

Abstract

It is required for a high throughput and accurate developmental toxicity test to evaluate a large number of chemicals. Developmental processes are regulated by interaction of signal transduction. Thus, we focused on the disruptions of these signalings caused by a chemical exposure in human iPS cells. Here, the disruption was monitored RTK-SRF signal which regulates limb/digit formation. The prediction showed high accuracy in the case of using chemicals which cause in limb/digit malformation. These results indicate signal disruption based test is useful for high throughput teratogenicity test.