Annual Meeting of the Japanese Society of Toxicology
The 48th Annual Meeting of the Japanese Society of Toxicology
Session ID : P-136
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Host: The Japanese Society of Toxicology

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Development of a new in vitro reporter system for detection of chemicals potentiating epigenetic toxicity on DNA methylation
*Maky I. OTSUKA, Misato MIYAI, Naoki YAMAMOTO, Ayaka TSUCHIMOTO, Hideki TAMURA, Kentaro TANEMURA, Makoto SHIBUTANI, Katsuhide IGARASHI
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Abstract

Epigenetic toxicity is thought to be caused by the disruption of epigenetic regulation due to chemical exposure. We constructed a novel in vitro system for detection of alterations in DNA methylation status by chemicals. In Neuro-2a, we introduced a reporter system by selecting Agouti-IAP and Daz1 as promoters. Using 5azaC, we found an increase in luciferase activity, suggesting a decrease in methylation. Pyrosequencing confirmed the decrease in DNA methylation. Thus, we could develop a convenient cellular system for detection of modifying effect of chemicals on DNA methylation status.

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