An attempt to develop the evaluation method for calcium transient in human iPS cell-derived cardiomyocyte without dedicated devices

Abstract

We attempted to develop the method for detecting cardiotoxicity in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) without dedicated devices. The toxicity of cardiotoxic compounds (sotalol, moxifloxacin, lidocaine, verapamil, pentamidine) were tested in spheroids of GCaMP-transfected hiPSC-CM by measuring calcium transient with a combination of commonly used imaging plate reader and open-sourced imaging software. The toxicity of hERG, Nav and Cav ion channel inhibitors, beta blocker and hERG channel trafficking inhibitor were able to be detected with our method.