Histopathological and immunohistochemical evaluation of changes in blood hormone levels induced by endocrine disruptors

Abstract

We performed immunohistochemical analysis to develop novel method for detection of endocrine disruptors. Aminotriazole (AMT), propylthiouracil (PTU), aminoglutethimide (AGT) and other 3 chemicals were administered to SD rats for 28 days. Decreases in serum T3 and T4 in AMT and PTU groups and an increase in serum TSH in AMT, PTU and AGT groups were detected. Immunostaining in these groups revealed a decrease in T4 in the thyroid gland and an increase in TSH positive area ratio in the anterior pituitary gland, suggesting the possibility of detecting hormonal changes using pathological specimens.