Suppression of PI3K–Akt signaling mediated by methyl vinyl ketone through protein modification

Abstract

Methyl vinyl ketone (MVK), an environmental electrophile, is known to interact with proteins via Michael reaction. In this study, we investigated the effect of MVK on phosphatidyl inositol-3 kinase (PI3K) –Akt signaling. We found that MVK attenuated PI3K–Akt pathway in a dose-dependent manner. Interestingly, MVK affected PI3K p85 subunit and inhibited to bind to epidermal growth factor receptor. These results indicated that modification of PI3K by MVK was involved in the regulation of PI3K–Akt signaling.