Effect of long-term culture on variation of chromosome number of clones isolated from thio-dimethylarsinic acid-exposed cells

Abstract

Thio-dimethylarsinic acid (thio-DMA^V), which is a pentavalent methyl arsenic compound in which the oxygen atom of dimethylarsinic acid (DMA^V) is substituted with a sulfur atom, has been identified in the human urine. Previously, we have showed 75% of clones have abnormal chromosome number in which isolated from the V79 cells exposed with 2.5 μM thio-DMA^V for 40 hours. In this study, we investigated the effects of long-term culture on chromosome number of each clones. Then we showed that some clones had indicated the different abnormal chromosome number after long-term culture.