Extrapolation of pseudo-allergic reactions seen in the dog toxicity study to humans - Evaluation by using MRGPRX2-expressed cells -

Abstract

MAS-related G protein coupled receptor-X2 (MRGPRX2) is a receptor expressed in human mast cells and associated with drug-induced pseudo-allergic reactions. In our experiments, dog MRGPRX2 was identified as the orthologue of human MRGPRX2, dog or human MRGPRX2-expressed cells could respond to histamine-releasing agents, and the sensitivity of the dog cells to those agents was higher than the human cells. MRGPRX2-expressed cells were considered useful to investigate the relevance of the reactions seen in dogs receiving a new compound to humans and the species difference of the sensitivity.