Host: The Japanese Society of Toxicology
Alkyne is a tiny functional group, which can be combined with various functional groups by click reaction, and now widely used as a tag for small compounds. Alkyne is also known to have a strong Raman signal in the cellular silent region, in which no endogenous molecules show Raman signal. Therefore, we applied Raman microscope for the detection of alkyne-tagged molecules and successfully established alkyne tag Raman imaging (ATRI). Recently, we also applied ATRI for the analysis of protein modifications by small compounds and developed a new method, alkyne-tag Raman screening (ATRaS). In this presentation, several examples of ATRI and ATRaS will be shown.
