Host: The Japanese Society of Toxicology
Name : The 49th Annual Meeting of the Japanese Society of Toxicology
Date : June 30, 2022 - July 02, 2022
[Introduction]
Tetrabromobisphenol A (TBBPA) and hexabromocyclododecane (HBCD) are widely used type of brominated flame retardant, which is employed as additives in the manufacturing of office and home electronic equipment. Although endocrine-disrupting effects of TBBPA and HBCD have been reported, information about neurotoxicity is limited. In this study, we investigated the effects of TBBPA and HBCD on necroptosis signaling in PC12 cells.
[Methods]
PC12 cells were treated with TBBPA and HBCD for 24 hours, and then cell viability was measured by CCK8 and LDH assay. The relative mRNA expression levels of RIPK1, RIPK3, and MLKL were determined by real-time RT-PCR. Protein levels of RIPK1, RIPK3, and MLKL were determined by Western blot analysis.
[Results and Discussion]
Cell viability was decreased after treatment with TBBPA and HBCD for 24 hours in a concentration-dependent manner. RIPK3 and MLKL mRNA and protein levels were significantly increased after treatment with TBBPA and HBCD, whereas changes in RIPK1 levels were minimal. Necrostatin-1, a necroptosis inhibitor, significantly inhibited the cytotoxicity induced by TBBPA and HBCD.
[Conclusions]
These results suggest that TBBPA and HBCD activate necroptosis signaling in PC12 cells.
