Host: The Japanese Society of Toxicology
Name : The 49th Annual Meeting of the Japanese Society of Toxicology
Date : June 30, 2022 - July 02, 2022
Occupational bladder cancer caused by aromatic amines is a social problem, and it has recently occurred in workers who handle aromatic amines such as o-toluidine (OTD) in Japan. We investigated the effects on the bladder urothelium by aromatic amines exposed at a chemical plant in Fukui Prefecture, and detected the correlation between the presence of OTD and its metabolites in urine and the proliferative effect on the bladder urothelium. In the present study, we investigated effects of the urinary OTD metabolites 2-amino-m-cresol (2AMC), 4-amino-m-cresol (4AMC) and o-acetotoluidine (AOTD) on the bladder epithelium. Male rats were treated with 2AMC, 4AMC or AOTD for 4 weeks. Incidences of simple hyperplasia and cell proliferation were induced by AOTD treatment in the bladder urothelium, but not by 2AMC or 4AMC treatment. There was no difference in TUNEL positivity among the groups. These results indicate that AOTD, a urinary metabolite of OTD-related aromatic amine exposure, may be involved in bladder carcinogenesis.
