Host: The Japanese Society of Toxicology
Name : The 50th Annual Meeting of the Japanese Society of Toxicology
Date : June 19, 2023 - June 21, 2023
Repair of DNA double-strand breaks (DSBs) by homologous recombination during meiosis is crucial for gametogenesis. RAD51 and DMC1 recombinases mediate strand exchange between homologous DNA, which facilitates synapsis of homologous chromosomes and repair of DSBs. Here we report that FIGNL1, a AAA+ ATPase, is indispensable for spermatogenesis in mice. Germ cell-specific conditional knockout of Fignl1 revealed accumulation of RAD51 and DMC1 in meiotic S-phase and early meiotic prophase I in a meiotic DSB-independent manner, consistent with an expected role of FIGNL1 as an anti-recombinase. Synapsis between homologous chromosomes is defective, and spermatocytes are eliminated before completion of meiotic prophase I in Fignl1 cKO mice. Our findings indicate essential roles of FIGNL1-dependent removal of recombinases in homologous recombination during mammalian gamatogenesis.
