Host: The Japanese Society of Toxicology
Name : The 51st Annual Meeting of the Japanese Society of Toxicology
Date : July 03, 2024 - July 05, 2024
【Background】Cholesteric cholelithiasis (CCT) is the most popular diseases in all of cholelithiasis (CT). It has been reported there are some functional changes of gall bladder (GB) as one of the factors for CT, but there has not been established the mechanism related to the functional changes of GB or the onset of gall bladder cancer (GBC). There has been some method to evaluate drug efficacy using mouse model, but in terms of very few GB tissue from mouse, and animal ethics, therefore more effective research model is required.
【Objectives】We focused on organoid culture method as in vitro culture model and developed “GB organoid derived from CCT model mouse” and clarified its usefulness.
【Methods】We fed CCT induced food to 8 weeks mice, and produced CCT model mice. Using extracted tissue, we evaluated the pathological characteristics about gall stones formation and GB structure. And also, we cultured GB organoid derived from extracted tissue and compared its structure or mucus production with original tissue. Additionally, the gene expression difference was analyzed by RNA sequence.
【Results】The gall stones were formed in induced food mouse. Organoid size was significantly bigger than control group and GB inflammation or wall hypertrophy was recognized in GB tissue. In RNA seq., some pathways related to the appearance of GBC was activated about induced food group.
【Conclusion】It was suggested that GB organoid derived from CCT model mouse can reproduce pathological characteristics of CT, and it was cleared that CT contributes to onset of GBC compared with normal organoid.
